| PL | Name | Time | H# | ||
| 1 |  | Maya Metivier Windsor Lancers | 41.36 | 4 | |
| 2 |  | Ella Steel-Douglas Border City Ac | 42.16 | 4 | |
| 3 |  | Madison Gravelle U Windsor AC | 43.19 | 4 | |
| 4 | | Marissa Madia Border City Ac | 43.44 | 3 | |
| 5 | | Julia Carswell Border City Ac | 43.46 | 2 | |
| 6 | | Lara Watchorn Windsor Lancers | 43.80 | 3 | |
| 7 | | Kaitlyn Gravelle U Windsor AC | 43.97 | 4 | |
| 8 |  | Katie Coenjarts Sarnia Asw | 44.42 | 2 | |
| 9 | | Gwendolyn Flanagan Border City Ac | 44.61 | 2 | |
| 10 | | Erin Bailey-Roy Border City Ac | 45.35 | 1 | |
| 11 |  | Mya Bart Windsor Legion | 46.91 | 3 | |
| 12 | | Isabella Madia Border City Ac | 47.78 | 2 | |
| 13 | | Kyelann Elliott Border City Ac | 48.66 | 1 | |
| 14 | | Aydalin Martinez-Wheeler Border City Ac | 48.77 | 1 | |
| 15 |  | Alexis Gray St Thomas Legion | 49.27 | 2 | |
| 16 | | Niyah McKay Border City Ac | 50.49 | 1 | |
| 17 | | Peyton Sexton-Jones Windsor Legion | 53.56 | 1 |