| Record | Time | Date | Name | |
| 7 | Advance | by | Time | -- |
| Sec | Lane | Name | Seed | ||
| 1 | 1 |
Empty | |||
| 1 | 2 | Catenacci, Cassie Windsor Legion | |||
| 1 | 3 | John, Aiyana St Thomas Legion | |||
| 1 | 4 | Gravelle, Kaitlyn U Windsor AC | |||
| 1 | 5 | Matenda, Davinence Windsor Legion | |||
| 1 | 6 | Gravelle, Madison U Windsor AC | |||
| 1 | 7 |
Empty |