| PL |  | Team | Time | H# | 
| 1 |  | Western Mustangs | 1:29.34 | 4 | 
|  |  | 1) Alex Collins | 2) Thomas Helland 44.527 |  |  | 
|  |  | 3) Aaron Thompson 22.321 | 4) Robbie Menzies 22.485 |  |  | 
| 2 |  | Guelph Gryphons | 1:29.35 | 4 | 
|  |  | 1) Travis Campbell | 2) Logan Spicer 44.487 |  |  | 
|  |  | 3) Carter Teal 22.098 | 4) Richard Davis 22.764 |  |  | 
| 3 |  | Waterloo Warriors | 1:31.72 | 2 | 
|  |  | 1) Dominik Manouchehr | 2) Owen Babcock 45.881 |  |  | 
|  |  | 3) Ben Lemon 23.000 | 4) Lucas Baranoski 22.834 |  |  | 
| 4 |  | Windsor Lancers  'B' | 1:32.60 | 2 | 
|  |  | 1) Cameron John-Dunslow | 2) Weagbe Mombo 45.846 |  |  | 
|  |  | 3) Ethan Myers 23.305 | 4) Amari Hernandez 23.442 |  |  | 
| 5 |  | Toronto | 1:33.09 | 4 | 
|  |  | 1) Logan Duley | 2) Brandon Tang 46.332 |  |  | 
|  |  | 3) Adam Exley 23.788 | 4) Caiden Schultz 22.963 |  |  | 
| 6 |  | Laurier Golden Hawks | 1:33.84 | 2 | 
|  |  | 1) Tyriq Quayson | 2) Jonah Lee 46.356 |  |  | 
|  |  | 3) Fabian Cox 23.421 | 4) Russell Allen 24.061 |  |  | 
| 7 |  | Toronto  'B' | x1:34.13 | 3 | 
|  |  | 1) David Adeleye | 2) Cameron Reyes 46.272 |  |  | 
|  |  | 3) Ryker Sung 23.461 | 4) Clarence Cheung 24.397 |  |  | 
| 8 |  | Toronto Metro Bold | 1:34.92 | 1 | 
|  |  | 1) Aaron Kelly | 2) Tafara Gwata 46.231 |  |  | 
|  |  | 3) Zugs Hirse 23.143 | 4) Seon Valentine 25.544 |  |  | 
| 9 |  | Waterloo Warriors  'B' | x1:35.29 | 1 | 
|  |  | 1) Aiden Parkins | 2) Ben Smith 46.682 |  |  | 
|  |  | 3) David Gbenjo 23.210 | 4) David Alabi 25.392 |  |  | 
| 10 |  | York Lions | 1:35.41 | 3 | 
|  |  | 1) Kejean Jackson | 2) Kasper Janicki 48.176 |  |  | 
|  |  | 3) David Asemota 23.920 | 4) Markmeena Makcemus 23.312 |  |  | 
| -- |  | Western Mustangs  'B' | DNF | 2 | 
|  |  | 1) Zach Theodore | 2) Kwame Karangwa 46.308 |  |  | 
|  |  | 3) Caleb Clarke 23.008 | 4) Adam Angerilli |  |  | 
| -- |  | Brock Badgers | DQ | 1 | 
|  |  | 1) Colton Spencer | 2) Tyrell Smikle 46.813 |  |  | 
|  |  | 3) Keshawn Igbinosun 23.070 | 4) Olobo Okpanachi 23.519 |  |  |