| Record | Time | Date | Name | |
| Meet: | 33.74 | 2020 | Daniel | Harper Guelph-- |
| USports: | 34.46 | -- | ||
| Sec | Lane | Name | Seed | ||
| 1 | 1 |
Empty | |||
| 1 | 2 | Tristan Foran Lakehead | 39.00 | ||
| 1 | 3 | Faiz Yessoufou York | 38.65 | ||
| 1 | 4 | Shun Chen Toronto | 38.50 | ||
| 1 | 5 | Karsten Brown Brock | 38.30 | ||
| 1 | 6 | Khalifa Badamasi Brock | 38.30 |
| Sec | Lane | Name | Seed | ||
| 2 | 1 |
Empty | |||
| 2 | 2 | Aidan Cowell-Miller Western | 37.96 | ||
| 2 | 3 | Tom Huynh Waterloo | 37.51 | ||
| 2 | 4 | Jaden McKenzie Detroit Mercy | 37.25 | ||
| 2 | 5 | Aiden Codrington York | 37.10 | ||
| 2 | 6 | Kaya Dizon-Spence Toronto | 37.20 |
| Sec | Lane | Name | Seed | ||
| 3 | 1 |
Empty | |||
| 3 | 2 |
Empty | |||
| 3 | 3 | Payton Norkey Detroit Mercy | 37.09 | ||
| 3 | 4 | Caleb Clarke Western | 37.01 | ||
| 3 | 5 | Tate Kwaiser Detroit Mercy | 37.00 | ||
| 3 | 6 | Michael Chinoda Windsor | 37.00 |
| Sec | Lane | Name | Seed | ||
| 4 | 1 |
Empty | |||
| 4 | 2 |
Empty | |||
| 4 | 3 | Nathan Maxey Windsor | 36.92 | ||
| 4 | 4 | David Gbenjo Waterloo | 36.90 | ||
| 4 | 5 | Pk Moyo Toronto | 36.80 | ||
| 4 | 6 | Auxanne Yavo Guelph | 36.86 |
| Sec | Lane | Name | Seed | ||
| 5 | 1 |
Empty | |||
| 5 | 2 |
Empty | |||
| 5 | 3 | Richard Neudorf York | 36.80 | ||
| 5 | 4 | Nick Mancini Brock | 36.80 | ||
| 5 | 5 | Vahaab Ladha Laurier | 36.68 | ||
| 5 | 6 | Carvin Coleman Detroit Mercy | 36.75 |
| Sec | Lane | Name | Seed | ||
| 6 | 1 |
Empty | |||
| 6 | 2 |
Empty | |||
| 6 | 3 | Anthony Isaac Windsor | 36.65 | ||
| 6 | 4 | Cameron Reyes Toronto | 36.58 | ||
| 6 | 5 | Ben Smith Waterloo | 36.53 | ||
| 6 | 6 | Jonah Lee Laurier | 36.56 |
| Sec | Lane | Name | Seed | ||
| 7 | 1 |
Empty | |||
| 7 | 2 |
Empty | |||
| 7 | 3 | Cameron John-Dunslow Windsor | 36.50 | ||
| 7 | 4 | Adam Exley Toronto | 36.50 | ||
| 7 | 5 | Kejean Jackson York | 36.30 | ||
| 7 | 6 | Dylen Belanger Western | 36.48 |
| Sec | Lane | Name | Seed | ||
| 8 | 1 |
Empty | |||
| 8 | 2 |
Empty | |||
| 8 | 3 | Keshawn Igbinosun Brock | 36.26 | ||
| 8 | 4 | Leewinchell Jean Windsor | 36.20 | ||
| 8 | 5 | Ryan Mahabir Windsor | 36.04 | ||
| 8 | 6 | Nicholas Williams Western | 36.14 |
| Sec | Lane | Name | Seed | ||
| 9 | 1 |
Empty | |||
| 9 | 2 |
Empty | |||
| 9 | 3 |
Empty | |||
| 9 | 4 | Antonio Talley Detroit Mercy | 35.76 | ||
| 9 | 5 | Liam Clark Waterloo | 35.57 | ||
| 9 | 6 | Luke Adamczyk Detroit Mercy | 35.72 |
| Sec | Lane | Name | Seed | ||
| 10 | 1 |
Empty | |||
| 10 | 2 |
Empty | |||
| 10 | 3 |
Empty | |||
| 10 | 4 | Aaron Thompson Waterloo | 35.43 | ||
| 10 | 5 | Carter Teal Guelph | 35.28 | ||
| 10 | 6 | Syelis Brooks Guelph | 35.38 |
| Sec | Lane | Name | Seed | ||
| 11 | 1 |
Empty | |||
| 11 | 2 |
Empty | |||
| 11 | 3 |
Empty | |||
| 11 | 4 | Richard Davis Guelph | 35.27 | ||
| 11 | 5 | Alex Collins Western | 35.02 | ||
| 11 | 6 | Robbie Menzies Western | 35.20 |