| Record | Time | Date | Name | |
| Meet: | 33.74 | 2020 | Daniel | Harper Guelph-- | 
| USports: | 34.46 | -- | ||
| Sec | Lane | Name | Seed | ||
| 1 | 1 | 
 Empty  | |||
| 1 | 2 | Tristan Foran Lakehead  | 39.00 | ||
| 1 | 3 | Faiz Yessoufou York  | 38.65 | ||
| 1 | 4 | Shun Chen Toronto  | 38.50 | ||
| 1 | 5 | Karsten Brown Brock  | 38.30 | ||
| 1 | 6 | Khalifa Badamasi Brock  | 38.30 | 
| Sec | Lane | Name | Seed | ||
| 2 | 1 | 
 Empty  | |||
| 2 | 2 | Aidan Cowell-Miller Western  | 37.96 | ||
| 2 | 3 | Tom Huynh Waterloo  | 37.51 | ||
| 2 | 4 | Jaden McKenzie Detroit Mercy  | 37.25 | ||
| 2 | 5 | Aiden Codrington York  | 37.10 | ||
| 2 | 6 | Kaya Dizon-Spence Toronto  | 37.20 | 
| Sec | Lane | Name | Seed | ||
| 3 | 1 | 
 Empty  | |||
| 3 | 2 | 
 Empty  | |||
| 3 | 3 | Payton Norkey Detroit Mercy  | 37.09 | ||
| 3 | 4 | Caleb Clarke Western  | 37.01 | ||
| 3 | 5 | Tate Kwaiser Detroit Mercy  | 37.00 | ||
| 3 | 6 | Michael Chinoda Windsor  | 37.00 | 
| Sec | Lane | Name | Seed | ||
| 4 | 1 | 
 Empty  | |||
| 4 | 2 | 
 Empty  | |||
| 4 | 3 | Nathan Maxey Windsor  | 36.92 | ||
| 4 | 4 | David Gbenjo Waterloo  | 36.90 | ||
| 4 | 5 | Pk Moyo Toronto  | 36.80 | ||
| 4 | 6 | Auxanne Yavo Guelph  | 36.86 | 
| Sec | Lane | Name | Seed | ||
| 5 | 1 | 
 Empty  | |||
| 5 | 2 | 
 Empty  | |||
| 5 | 3 | Richard Neudorf York  | 36.80 | ||
| 5 | 4 | Nick Mancini Brock  | 36.80 | ||
| 5 | 5 | Vahaab Ladha Laurier  | 36.68 | ||
| 5 | 6 | Carvin Coleman Detroit Mercy  | 36.75 | 
| Sec | Lane | Name | Seed | ||
| 6 | 1 | 
 Empty  | |||
| 6 | 2 | 
 Empty  | |||
| 6 | 3 | Anthony Isaac Windsor  | 36.65 | ||
| 6 | 4 | Cameron Reyes Toronto  | 36.58 | ||
| 6 | 5 | Ben Smith Waterloo  | 36.53 | ||
| 6 | 6 | Jonah Lee Laurier  | 36.56 | 
| Sec | Lane | Name | Seed | ||
| 7 | 1 | 
 Empty  | |||
| 7 | 2 | 
 Empty  | |||
| 7 | 3 | Cameron John-Dunslow Windsor  | 36.50 | ||
| 7 | 4 | Adam Exley Toronto  | 36.50 | ||
| 7 | 5 | Kejean Jackson York  | 36.30 | ||
| 7 | 6 | Dylen Belanger Western  | 36.48 | 
| Sec | Lane | Name | Seed | ||
| 8 | 1 | 
 Empty  | |||
| 8 | 2 | 
 Empty  | |||
| 8 | 3 | Keshawn Igbinosun Brock  | 36.26 | ||
| 8 | 4 | Leewinchell Jean Windsor  | 36.20 | ||
| 8 | 5 | Ryan Mahabir Windsor  | 36.04 | ||
| 8 | 6 | Nicholas Williams Western  | 36.14 | 
| Sec | Lane | Name | Seed | ||
| 9 | 1 | 
 Empty  | |||
| 9 | 2 | 
 Empty  | |||
| 9 | 3 | 
 Empty  | |||
| 9 | 4 | Antonio Talley Detroit Mercy  | 35.76 | ||
| 9 | 5 | Liam Clark Waterloo  | 35.57 | ||
| 9 | 6 | Luke Adamczyk Detroit Mercy  | 35.72 | 
| Sec | Lane | Name | Seed | ||
| 10 | 1 | 
 Empty  | |||
| 10 | 2 | 
 Empty  | |||
| 10 | 3 | 
 Empty  | |||
| 10 | 4 | Aaron Thompson Waterloo  | 35.43 | ||
| 10 | 5 | Carter Teal Guelph  | 35.28 | ||
| 10 | 6 | Syelis Brooks Guelph  | 35.38 | 
| Sec | Lane | Name | Seed | ||
| 11 | 1 | 
 Empty  | |||
| 11 | 2 | 
 Empty  | |||
| 11 | 3 | 
 Empty  | |||
| 11 | 4 | Richard Davis Guelph  | 35.27 | ||
| 11 | 5 | Alex Collins Western  | 35.02 | ||
| 11 | 6 | Robbie Menzies Western  | 35.20 |