| Record | Flag | Time/mark | Date | |
| Meet: | * | 33.89 | 1993 | Anthony Price-- |
| USports: | ! | 34.46 | -- | |
| PL | Name | Time | H# | ||
| 1 | Emmett Bravakis Toronto | 34.75 | |||
| 2 | Craig Thorne Guelph Gryphons | 35.20 | |||
| 3 | Ben Tilson Windsor Lancers | 35.46 | |||
| 4 | Kobie Warden Windsor Legi | 35.63 | 9 | ||
| 5 | Alexander Di Florio Centro Scuol | 35.83 | |||
| 6 | Richard Davis Guelph Gryphons | 35.86 | |||
| 7 | Syelis Brooks Guelph Gryphons | 35.97 | |||
| 8 | Aaron Thompson Waterloo Warriors | 36.02 | |||
| 9 | Carter Teal Guelph Gryphons | 36.10 | |||
| 10 | Liam Clark Waterloo Warriors | 36.16 | 8 | ||
| 11 | Caiden Schultz Toronto | 36.23 | |||
| 12 | Keshawn Igbinosun Brock Badgers | 36.29 | |||
| 13 | Luis Koidjos Lakehead | 36.42 | 7 | ||
| 14 | Ayden Blain Western Mustangs | 36.56 | 6 | ||
| 15 | Ryan Gilham Windsor Lancers | 36.62 | 8 | ||
| 16 | Lucas Baranoski Waterloo Warriors | 36.88 | 7 | ||
| 17 | Ben Lemon Waterloo Warriors | 36.91 | 4 | ||
| 18 | Auxane Yao Guelph Gryphons | 37.09 | |||
| 19 | Matthew MacNeill Guelph Gryphons | 37.12 | |||
| 20 | Ben Smith Waterloo Warriors | 37.14 | 8 | ||
| 21 | Jonah Lee Laurier | 37.17 | 9 | ||
| 22 | Tyrell Smikle Brock Badgers | 37.25 | 5 | ||
| 23 | Nicholas Carson-Pedri Lakehead | 37.29 | 4 | ||
| 24 | Dylen Belanger Western Mustangs | 37.30 | 6 | ||
| 25 | Nick Mancini Brock Badgers | 37.31 | 5 | ||
| 26 | Richard Neudorf York Lions | 37.41 | 4 | ||
| 27 | Nathan Maxey Windsor Lancers | 37.54 | 7 | ||
| 28 | Aidan Cowell-Miller Western Mustangs | 37.96 | 3 | ||
| 29 | Aiden Codrington York Lions | 38.23 | 2 | ||
| 30 | Jonas Schaefer Brock Badgers | 38.29 | 1 | ||
| 31 | Kejean Jackson York Lions | 38.32 | 7 | ||
| 32 | Tom Huynh Waterloo Warriors | 38.42 | 3 | 38.415 | |
| 33 | Ronan Radovich Windsor Lancers | 38.94 | 4 | ||
| 34 | Khalifa Badamasi Brock Badgers | 39.33 | 1 | ||
| 35 | Karsten Brown Brock Badgers | 39.56 | 2 | ||
| 36 | Kyle Clahar Windsor Lancers | 39.98 | 3 | ||
| 37 | Kareem Ramadan UTTC | 40.27 | 2 | ||
| 38 | Joshua Roberto - VI I Be Fast | 43.04 | 1 | ||
| -- | Abraham Ater Centro Scuol | DNF | 13 | ||
| -- | Owen Yates Brock Badgers | DQ | |||
| -- | David Gbenjo Waterloo Warriors | DQ |