| PL |
Name |
Time |
H# |
|
| 1 | Hutchins, Aleeya 01 The Gazelles | 56.80Q | 2 | |
| 2 | Colaire, Shaleah 01 Durham Legion | 57.17Q | 4 | |
| 3 | Gibson, Kiara 01 C.A.N.I. Athletics | 57.30Q | 3 | |
| 4 | Stieh, Amy 01 Lakehead Running | 57.51Q | 1 | |
| 5 | Watson, Camille 01 Speed Academy | 57.49q | 3 | |
| 6 | Jovic, Helena 01 Ottawa Lions T.F.C. | 57.88q | 3 | |
| 7 | Okpali, Favour 01 Uni. Toronto TC | 57.91q | 4 | |
| 8 | Larochelle, Clémence 01 Dynamique de Laval | 58.70q | 2 | |
| 9 | Erotocritou, Andriana 03 The Gazelles | 59.06 | 1 | |
| 10 | Parsons, Nicole 00 Oakville Legion | 59.28 | 2 | |
| 11 | Jacobs, Lauren 02 Toronto West | 59.66 | 3 | |
| 12 | Hempstead, Lucy 01 C.A.N.I. Athletics | 59.93 | 2 | |
| 13 | Heath, Jasmine 03 Game Speed Int. | 1:00.09 | 3 | |
| 14 | McDermott, Brynley 03 Speed Academy | 1:00.54 | 2 | |
| 15 | St. Louis, Madison 03 Durham Legion | 1:00.74 | 4 | |
| 16 | Matthews, Amelia 02 Speed River | 1:00.84 | 1 | |
| 17 | Peddie, Alysha 03 Game Speed Int. | 1:01.22 | 2 | |
| 18 | Campeau, Kennedy 00 Border City AC | 1:01.29 | 1 | |
| 19 | La-Rose, Arden 03 Central Toronto | 1:01.45 | 2 | |
| 20 | Custeau, Jade 01 Zénix | 1:01.70 | 4 | |
| 21 | Boisclair, Rosalie 01 Zénix | 1:01.74 | 1 | |
| 22 | Lanctôt, Laurence 00 Perfmax-Racing | 1:01.76 | 4 | |
| 23 | Somerville, Khalis 03 Speed Academy | 1:01.95 | 4 | |
| 24 | Kraftcheck, Sage 01 Hamilton Olympic | 1:04.14 | 3 | |
| 25 | Torrance, Megan 01 Blue Devils | 1:05.10 | 4 | |
| 26 | Brooks, Nayla 02 Speed Academy | 1:05.36 | 3 | |
| 27 | Wilson, Cora 01 South Simcoe | 1:05.89 | 3 | |
| 28 | Morales, Alexandra 02 Speed Academy | 1:06.30 | 1 | |
| 29 | Vangaalen, Imani 04 Kitchener T.F.A. | 1:06.40 | 1 | |
| 30 | Valeri, Sabrina 01 Centro Scuola | 1:06.51 | 2 | |
| -- | Heath, Jadyn 01 Game Speed Int. | DQ | | |