| PL |
Name |
Time |
Wind |
H# |
|
| 1 | Camille Watson 01 The Speed Academy | 25.24 | -1.3 | 7 | 10 |
| 2 | Anna Witherspoon 01 The Speed Academy | 25.24 | -1.3 | 7 | |
| 3 | Kayla Vieux 01 C.A.N.I. Athletics | 25.71 | -1.3 | 7 | |
| 4 | Christina Robert 01 Track North | 25.84 | -1.9 | 6 | |
| 5 | Krystalann Bechard 02 Border City AC | 26.03 | -1.9 | 6 | |
| 6 | Jermaila Bruce 01 C.A.N.I. Athletics | 26.04 | -1.3 | 7 | |
| 7 | Milenne Habash 02 Speed River | 26.14 | -1.3 | 7 | |
| 8 | Shaleah Colaire 01 Durham Legion | 26.15 | -1.3 | 7 | |
| 9 | Jazmine Oliver 01 Game Speed | 26.36 | -1.3 | 7 | |
| 10 | Georgina Pickard 02 Brantford T.F.C. | 26.38 | -1.3 | 7 | |
| 11 | Kyra Maloney 02 The Gazelles | 26.39 | -1.9 | 6 | |
| 12 | Paulina Procyk 02 Ottawa Lions T.F.C. | 26.42 | -1.9 | 6 | |
| 13 | Dacey Richmond 01 Kingston T.F.C. | 26.44 | -1.9 | 6 | |
| 14 | Janay Chapman 02 The Gazelles | 26.45 | -1.3 | 5 | |
| 15 | Sabrena Small 01 London Western | 26.47 | -1.3 | 5 | |
| 16 | Korina Black 01 Etobicoke T.F.C. | 26.73 | -1.8 | 4 | 26.721 |
| 16 | Amelia Matthews 02 Laurel Creek T.F.C. | 26.73 | -1.9 | 6 | 26.721 |
| 18 | Lyric Sargeant 01 Invictus AC | 26.84 | -1.3 | 5 | 26.832 |
| 19 | Doyin Ogunremi 02 Ottawa Lions T.F.C. | 26.84 | -1.9 | 6 | 26.839 |
| 20 | Keena Browne 01 Durham Legion | 26.89 | -1.8 | 4 | |
| 21 | Alexzandra Throndson 01 Univ. Toronto TC | 26.94 | -1.3 | 5 | |
| 22 | Aliyah Logan 02 The Speed Academy | 26.96 | -1.3 | 5 | |
| 23 | Breah Walls 01 Border City AC | 27.02 | -1.8 | 4 | |
| 24 | Collette Wolfe 02 Laurel Creek T.F.C. | 27.27 | -1.3 | 5 | |
| 25 | Daneille Lynch 01 Durham Legion | 27.46 | -1.8 | 4 | |
| 26 | Bethany Suen 01 York Univ. TC | 27.56 | -1.8 | 4 | |
| 27 | Megan Torrance 01 Blue Devils | 27.57 | -1.3 | 5 | |
| 28 | Kadiatou Wann 01 C.A.N.I. Athletics | 27.58 | -0.6 | 1 | |
| 29 | Gemiah Lewis 02 Terminal Velocit | 27.59 | -1.8 | 4 | |
| 30 | Mikayla Moffett 02 Univ. Toronto TC | 27.60 | -1.8 | 4 | |
| 31 | Jadyn Heath 01 Game Speed | 27.81 | -1.9 | 3 | |
| 32 | Thi-Anne Hillyard 02 Oakville Legion | 27.96 | -1.3 | 5 | |
| 33 | Bianca Belfon 01 The Gazelles | 28.17 | -3.8 | 2 | |
| 34 | Chiara Price 02 Univ. Toronto TC | 28.30 | -1.9 | 3 | |
| 35 | Hanna Schroeder 02 Border City AC | 28.43 | -0.6 | 1 | |
| 36 | Alexandra Morales 02 The Speed Academy | 28.51 | -1.9 | 3 | |
| 37 | Simone Timm 02 York Univ. TC | 28.53 | -1.9 | 3 | |
| 38 | Monaye Mitchell 01 Terminal Velocit | 28.70 | -1.9 | 3 | |
| 39 | Noelle Divakou 05 Ottawa Lions T.F.C. | 28.91 | -1.9 | 3 | |
| 40 | Brooklyne Moore 02 Timmins Porcupin | 29.03 | -3.8 | 2 | |
| 41 | Amanda Rosati 01 Etobicoke T.F.C. | 29.08 | -0.6 | 1 | |
| 42 | Luiza Lena 02 Etobicoke T.F.C. | 29.27 | -1.8 | 4 | |
| 43 | Shantay Morgan 02 York Univ. TC | 29.44 | -3.8 | 2 | 29.436 |
| 44 | Princess Parris 01 The Gazelles | 29.44 | -1.9 | 3 | 29.437 |
| 45 | Sage Kraftcheck 01 Hamilton Olympic | 29.58 | -1.9 | 3 | |
| 46 | Junia Campbell-Clarke 02 Terminal Velocit | 29.78 | -0.6 | 1 | |
| 47 | Amanda Sugimura 02 Mississauga T.F.C. | 30.41 | -3.8 | 2 | |
| 48 | Erika Aguilera Ballester 02 Niagara Olympic 31.14 -3.8 2 |
| | | |
| 49 | Celeste Hivon 02 Speed River | 31.60 | -3.8 | 2 | |
| -- | Jadyn Zeppa 02 Sault Athletics | DQ | -1.9 | 6 | 163.3a |